ratio-imaging fluorescence microscopy
- ratio-imaging fluorescence microscopy
-
A method of measurement of intracellular
pH or intracellular calcium levels, using
a fluorescent
probe molecule (see
fura-2), in which the two different excitation wavelengths are used, and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level, and emission at the other wavelength is not, then standardization for intracellular
probe concentration, efficiency of light collection,
inactivation of
probe and thickness of
cytoplasm can all be performed automatically.
Dictionary of molecular biology.
2004.
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